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Effects of sciatic-conditioned medium on neonatal rat retinal cells in vitro BJMBR
Torres,P.M.M.; Guilarducci,C.V.V.; Araujo,E.G..
Schwann cells produce and release trophic factors that induce the regeneration and survival of neurons following lesions in the peripheral nerves. In the present study we examined the in vitro ability of developing rat retinal cells to respond to factors released from fragments of sciatic nerve. Treatment of neonatal rat retinal cells with sciatic-conditioned medium (SCM) for 48 h induced an increase of 92.5 ± 8.8% (N = 7 for each group) in the amount of total protein. SCM increased cell adhesion, neuronal survival and glial cell proliferation as evaluated by morphological criteria. This effect was completely blocked by 2.5 µM chelerythrine chloride, an inhibitor of protein kinase C (PKC). These data indicate that PKC activation is involved in the effect...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cytokines; Neurotrophic factors; Retina; Schwann cells; Sciatic nerve.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100012
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Non-neuronal cells are not the limiting factor for the low axonal regeneration in C57BL/6J mice BJMBR
Oliveira,A.L.R.; Langone,F..
Peripheral axonal regeneration was investigated in adult male mice of the C57BL/6J (C), BALB/cJ (B) and A/J (A) strains and in their F1 descendants using a predegenerated nerve transplantation model. Four types of transplants were performed: 1) isotransplants between animals of the C, B and A strains; 2) donors of the C strain and recipients of the C x B and C x A breeding; 3) donors of the B strain and recipients of the C x B breeding, and 4) donors of the A strain and recipients of the C x A breeding. Donors had the left sciatic nerve transected and two weeks later a segment of the distal stump was transplanted into the recipient. Four weeks after transplantation the regenerated nerves were used to determine the total number of regenerated myelinated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nerve regeneration; Schwann cells; Isogenic mice; Nerve transplantation; Tubulization.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200011
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The neural crest stem cells: control of neural crest cell fate and plasticity by endothelin-3 Anais da ABC (AABC)
DUPIN,ELISABETH; REAL,CARLA; LeDOUARIN,NICOLE.
How the considerable diversity of neural crest (NC)-derived cell types arises in the vertebrate embryo has long been a key question in developmental biology. The pluripotency and plasticity of differentiation of the NC cell population has been fully documented and it is well-established that environmental cues play an important role in patterning the NC derivatives throughout the body. Over the past decade, in vivo and in vitro cellular approaches have unravelled the differentiation potentialities of single NC cells and led to the discovery of NC stem cells. Although it is clear that the final fate of individual cells is in agreement with their final position within the embryo, it has to be stressed that the NC cells that reach target sites are pluripotent...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neural crest; Avian embryo; Cell differentiation; Endothelin3; Peripheral nervous system; Melanocytes; Schwann cells; Mesectoderm.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652001000400007
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